A simple, specific, precise and accurate method development and validation of amiloride hydrochloride and torsemide tablet formulation by RP-HPLC was developed. The method employed BDS hypersil C18, 250 mm × 4.6 mm, 5µ (particle size), column as stationary phase. The mobile phase consisted of phosphate buffer and methanol (50:50 v/v) pH 4.0 adjusted by orthophosphoric acid, at a flow rate of 1 ml/min. Detection was carried out at wavelength 327 nm. The retention time of amiloride hydrochloride and torsemide were found to be 3.5 and 5.27 minutes respectively. Linearity was established by over the concentration range of 5 to 15 μg/ml for amiloride hydrochloride with correlation coefficients (r2) 0.997 and 10 to 30 μg/ml for torsemide with correlation coefficients (r2) 0.999. The percentage recovery obtained for amiloride hydrochloride and torsemide were 99.66% and 100% respectively. The method has been validated as per ICH guideline and successfully applied to estimation of amiloride hydrochloride and torsemide in tablet dosage form.
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